Acoustofluidics 10: scaling laws in acoustophoresis.

Measuring acoustic energy density in microchannel acoustophoresis using a simple and rapid light-intensity method.

We present a simple and rapid method for measuring the acoustic energy density in microchannel acoustophoresis based on light-intensity measurements of a suspension of particles. The method relies on the assumption that each particle in the suspension undergoes single-particle acoustophoresis. It is validated by the single-particle tracking method, and we show by proper re-scaling that the re-s...

Forthcoming Lab on a Chip tutorial series on acoustofluidics: acoustofluidics-exploiting ultrasonic standing wave forces and acoustic streaming in microfluidic systems for cell and particle manipulation.

Acoustofluidics 18: Microscopy for acoustofluidic micro-devices.

In this tutorial review in the thematic series "Acoustofluidics", we discuss the implementation and practice of optical microscopy in acoustofluidic micro-devices. Examples are given from imaging of acoustophoretic manipulation of particles and cells in microfluidic channels, but most of the discussion is applicable to imaging in any lab-on-a-chip device. The discussion includes basic principle...

Affinity-Bead-Mediated Enrichment of CD8+ Lymphocytes from Peripheral Blood Progenitor Cell Products Using Acoustophoresis

Acoustophoresis is a technique that applies ultrasonic standing wave forces in a microchannel to sort cells depending on their physical properties in relation to the surrounding media. Cell handling and separation for research and clinical applications aims to efficiently separate specific cell populations. Here, we investigated the sorting of CD8 lymphocytes from peripheral blood progenitor ce...

Surface acoustic wave (SAW) acoustophoresis: now and beyond.

On-chip manipulation of micro-objects has long been sought to facilitate fundamental biological studies and point-of-care diagnostic systems. In recent years, research on surface acoustic wave (SAW) based micro-object manipulation (i.e., SAW acoustophoresis) has gained significant momentum due to its many advantages, such as non-invasiveness, versatility, simple fabrication, easy operation, and...